CLCA1 Protein (AA 416-670) (His tag)

Catalog No. ABIN6239825

This not_set CLCA1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CLCA1 Protein (AA 416-670) (His tag) (ABIN6239825)

Target: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 97 %

Product Details

Protein Characteristics: AA 416-670

Purification tag / Conjugate: This CLCA1 protein is labelled with His tag.

Characteristics: Tag location: N-terminal His Tag

Biological Activity Comment: CLCA1 (Calcium-activated chloride channel regulator 1) is a member of the calcium sensitive chloride conductance protein family. This protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits. It has been reported that CLCA1 activates calcium-dependent chloride channel through the interaction with TMEM16A (anoctamin-1). Besides, there exits similarities between human and mouse TMEM16A in amino acid sequence with the identity of 89.66%. Thus, a functional ELISA assay was conducted to detect the association of recombinant human CLCA1 with recombinant mouse TMEM16A. Briefly, CLCA1 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TMEM16A-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CLCA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CLCA1 with TMEM16A was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of CLCA1 with TMEM16A

Application Details

Application Notes: Isoelectric Point: 7.1

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: CLCA1 (Chloride Channel Accessory 1 (CLCA1))

Background: Alternative Names: CaCC1, CLCRG1, Chloride Channel,Calcium Activated,Family Member 1, Calcium-activated chloride channel protein 1

Molecular Weight: 28kDa

UniProt: A8K7I4