Vitamin D Receptor Protein (VDR) (AA 272-427) (His tag)

Catalog No. ABIN6239849

This Vitamin D Receptor protein is produced in Escherichia coli (E. coli).

Quick Overview for Vitamin D Receptor Protein (VDR) (AA 272-427) (His tag) (ABIN6239849)

Target: Vitamin D Receptor (VDR)

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 90 %

Product Details

Protein Characteristics: AA 272-427

Purification tag / Conjugate: This Vitamin D Receptor protein is labelled with His tag.

Characteristics: Tag location: N-terminal His Tag

Biological Activity Comment: Vitamin D Receptor (VDR) is the nuclear hormone receptor for vitamin D3. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. It mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Besides, E1A binding protein p300 (EP300) has been identified as an interactor of VDR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VDR and recombinant human EP300. Briefly, VDR were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C . Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of VDR and EP300 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of VDR with EP300.

Application Details

Application Notes: Isoelectric Point: 6.5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 100 mMNaHCO₃, 500 mMNaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: Vitamin D Receptor (VDR)

Target Type: Chemical

Background: Alternative Names: NR1I1, Nuclear Receptor Subfamily 1 Group I Member 1, Calcitriol Receptor, 1,25-dihydroxyvitamin D3 receptor

Molecular Weight: 20kDa

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway