Interferon gamma Protein (IFNG) (AA 24-166) (His tag)

Catalog No. ABIN7278709

Recombinant Interferon gamma protein expressed in Escherichia coli (E. coli).

Quick Overview for Interferon gamma Protein (IFNG) (AA 24-166) (His tag) (ABIN7278709)

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 24-166

Purification tag / Conjugate: This Interferon gamma protein is labelled with His tag.

Purpose: IFN-gamma (human) (rec.) (His)

Specificity: Human IFN-gamma (aa 24-166) is fused at the C-terminus to a His-tag.

Characteristics: Protein. Human IFN-gamma (aa 24-166) is fused at the C-terminus to a His-tag. Source: E. coli. Endotoxin content: <0.1EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in 50  mM Tris-HCl, 400nM NaCl,  pH 8.0. Purity: >95 % (SDS-PAGE). IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six alpha-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

Endotoxin Level: <0.1EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV) .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in 50  mM Tris-HCl, 400nM NaCl,  pH 8.0.

Handling Advice: Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Background:

Alternate Names/Synonyms: Interferon-gamma IFNG

Product Description: IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six alpha-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

Molecular Weight: ~17kDa (SDS-PAGE)

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy