CD86 Protein (CD86) (AA 26-245) (Fc Tag)

Catalog No. ABIN6253424

Recombinant CD86 protein expressed in CHO Cells.

Quick Overview for CD86 Protein (CD86) (AA 26-245) (Fc Tag) (ABIN6253424)

Target: CD86

Protein Type: Recombinant

Origin: Mouse

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 26-245

Purification tag / Conjugate: This CD86 protein is labelled with Fc Tag.

Purpose: CD86 (mouse):Fc (mouse) (rec.)

Specificity: The extracellular domain of mouse CD86 (aa 26-245) is fused to the N-terminus of the Fc region of mouse IgG2a.

Characteristics: Protein. The extracellular domain of mouse CD86 (aa 26-245) is fused to the N-terminus of the Fc region of mouse IgG2a. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). CD86 (B7-2) is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells and monocytes as well as on vascular endothelial cells. B7-2 and the closely related CD80 (B7-1) exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent costimulation. B7-1 and B7-2 additionally bind the CD28-related protein CTLA-4, which is up-regulated and recruited to the immunological synapse (IS) at the onset of T cell activation. CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function. B7-2 is expressed earlier than B7-1 following APC activation and both proteins bind with higher affinity to CTLA-4 than to CD28. B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS. The relative participation of B7-1 and B7-2 in T cell costimulation can also alter the Th1/Th2 bias of the immune response. Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses.

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: CD86

Alternative Name: CD86

Background:

Alternate Names/Synonyms: B7-2, B70, ETC-1

Product Description: CD86 (B7-2) is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells and monocytes as well as on vascular endothelial cells. B7-2 and the closely related CD80 (B7-1) exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent costimulation. B7-1 and B7-2 additionally bind the CD28-related protein CTLA-4, which is up-regulated and recruited to the immunological synapse (IS) at the onset of T cell activation. CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function. B7-2 is expressed earlier than B7-1 following APC activation and both proteins bind with higher affinity to CTLA-4 than to CD28. B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS. The relative participation of B7-1 and B7-2 in T cell costimulation can also alter the Th1/Th2 bias of the immune response. Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses.

NCBI Accession: NP_062261

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation