HpARI (AA 17-251) protein (His tag)

Catalog No. ABIN6253463

Recombinant HpARI protein expressed in HEK-293 Cells.

Quick Overview for HpARI (AA 17-251) protein (His tag) (ABIN6253463)

Target: HpARI

Protein Type: Recombinant

Origin: Human, Mouse

Source: HEK-293 Cells

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 17-251

Purification tag / Conjugate: His tag

Purpose: HpARI (Alarmin Release Inhibitor) (rec.) (His)

Specificity: Heligmosomoides polygyrus protein HpARI (aa 17-251) is fused at the C-terminus to a His-tag.

Cross-Reactivity: Human, Mouse

Characteristics: Heligmosomoides polygyrus protein HpARI (aa 17-251) is fused at the C-terminus to a His-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: HpARI

Background:

Heligmosomoides Polygyrus Alarmin Release Inhibitor

HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. HpARI is a new type of reagent to prevent IL-33-mediated pathology.

Molecular Weight: ~40kDa (SDS-PAGE), ~40 kDa (Monomer) (SEC)