Flagellin Protein (FliC) (Mutant) (His tag)

Catalog No. ABIN6253486

This Recombinant Flagellin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Flagellin Protein (FliC) (Mutant) (His tag) (ABIN6253486)

Target: Flagellin (FliC)

Protein Type: Recombinant

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: Mutant

Purification tag / Conjugate: This Flagellin protein is labelled with His tag.

Purpose: Flagellin (TLR5 Mutant) (rec.)

Specificity: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.

Cross-Reactivity: Human, Mouse

Characteristics: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Flagellin (FliC)

Alternative Name: Flagellin

Background:

FliC (TLR5 Mutant)

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (TLR5 Mutant) (rec.) cannot be detected by human and mouse TLR5. It has not been tested on NLRC4.

Pathways: Inflammasome