NAD Nucleotidase Protein

Catalog No. ABIN6253506

This Recombinant NAD Nucleotidase protein is produced in Escherichia coli (E. coli).

Quick Overview for NAD Nucleotidase Protein (ABIN6253506)

Target: NAD Nucleotidase

Protein Type: Recombinant

Origin: Haemophilus influenzae

Source: Escherichia coli (E. coli)

Purity: >98 % (SDS-PAGE)

Product Details

Purpose: NAD Nucleotidase (H. influenzae) (rec.)

Specificity: Full-length NAD 5'-nucleotidase (Haemophilus influenzae) without the signal peptide corresponding to the first 26 aa.

Characteristics: Full-length NAD 5'-nucleotidase (Haemophilus influenzae) without the signal peptide corresponding to the first 26 aa.

Biological Activity Comment: With AMP: 856.4 nmol of Ade/min/mg of protein With NMN: 110,3 nmol of NR/min/mg of protein With NAD: 5.4 nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: 0.5 mg/mL (Lot specific)

Concentration: Lot specific

Buffer: In 5 mM KH2PO4, pH 6.8 containing 100 mM TRIS-HCl ( pH 8.5) and 10 mM MgCl2.

Handling Advice: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: NAD Nucleotidase

Background:

NadN, NAD Pyrophosphatase/5'-nucleotidase

NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi

Molecular Weight: ~63.0kDa

UniProt: Q4QNY4