Crk Protein (AA 1-204) (His tag)

Catalog No. ABIN6386629

Recombinant Crk protein expressed in Escherichia coli (E. coli).

Quick Overview for Crk Protein (AA 1-204) (His tag) (ABIN6386629)

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-204

Purification tag / Conjugate: This Crk protein is labelled with His tag.

Sequence: MAGNFDSEER SSWYWGRLSR QEAVALLQGQ RHGVFLVRDS STSPGDYVLS VSENSRVSHY IINSSGPRPP VPPSPAQPPP GVSPSRLRIG DQEFDSLPAL LEFYKIHYLD TTTLIEPVSR SRQGSGVILR QEEAEYVRAL FDFNGNDEED LPFKKGDILR IRDKPEEQWW NAEDSEGKRG MIPVPYVEKY RPASASVSAL IGGR

Characteristics: CRK, 1-204aa, Human, His tag, E.coli

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: Crk

Background: CRK is a member of signaling adapter protein family that binds to several tyrosine-phosphorylated proteins. This protein is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an SH2 followed by two SH3 domains (src-homology domains). The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Recombinant human CRK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molecular Weight: 25.0 kDa (224aa) confirmed by MALDI-TOF

NCBI Accession: NP_005197

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor