GALT Protein (AA 1-379) (His tag)
Quick Overview for GALT Protein (AA 1-379) (His tag) (ABIN6387110)
Target
See all GALT ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-379
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Purification tag / Conjugate
- This GALT protein is labelled with His tag.
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Sequence
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MSRSGT DPQQRQQASE ADAAAATFRA NDHQHIRYNP LQDEWVLVSA HRMKRPWQGQ VEPQLLKTVP RHDPLNPLCP GAIRANGEVN PQYDSTFLFD NDFPALQPDA PSPGPSDHPL FQAKSARGVC KVMCFHPWSD VTLPLMSVPE IRAVVDAWAS VTEELGAQYP WVQIFENKGA MMGCSNPHPH CQVWASSFLP DIAQREERSQ QAYKSQHGEP LLMEYSRQEL LRKERLVLTS EHWLVLVPFW ATWPYQTLLL PRRHVRRLPE LTPAERDDLA SIMKKLLTKY DNLFETSFPY SMGWHGAPTG SEAGANWNHW QLHAHYYPPL LRSATVRKFM VGYEMLAQAQ RDLTPEQAAE RLRALPEVHY HLGQKDRETA TIA -
Purification
- purified by chromatography
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Galactose-1-Phosphate Uridylyltransferase (GALT) (AA 1-379) protein (His tag)
custom-made GALT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Galactose-1-Phosphate Uridylyltransferase (GALT) (AA 1-379) protein (His tag)custom-made GALT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Galactose-1-Phosphate Uridylyltransferase (GALT) (AA 1-379) protein (GST tag)GALT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Galactose-1-Phosphate Uridylyltransferase (GALT) proteinGALT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % pure SDS
Galactose-1-Phosphate Uridylyltransferase (GALT) (AA 1-379), (full length) protein (His-SUMO Tag)GALT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Galactose-1-Phosphate Uridylyltransferase (GALT) protein (Myc-DYKDDDDK Tag)GALT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer (pH 8.5) containing 0.2 M NaCl, 10% glycerol.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
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Alternative Name
- GALT
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Background
- GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
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Molecular Weight
- 45.9 kDa (403aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000146
Target
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