LPCAT1 Protein (AA 79-534) (His tag)
Quick Overview for LPCAT1 Protein (AA 79-534) (His tag) (ABIN6387148)
Target
See all LPCAT1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 79-534
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Purification tag / Conjugate
- This LPCAT1 protein is labelled with His tag.
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Sequence
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SAEKEPE QPPALWRKVV DFLLKAIMRT MWFAGGFHRV AVKGRQALPT EAAILTLAPH SSYFDAIPVT MTMSSIVMKA ESRDIPIWGT LIQYIRPVFV SRSDQDSRRK TVEEIKRRAQ SNGKWPQIMI FPEGTCTNRT CLITFKPGAF IPGAPVQPVV LRYPNKLDTI TWTWQGPGAL EILWLTLCQF HNQVEIEFLP VYSPSEEEKR NPALYASNVR RVMAEALGVS VTDYTFEDCQ LALAEGQLRL PADTCLLEFA RLVRGLGLKP EKLEKDLDRY SERARMKGGE KIGIAEFAAS LEVPVSDLLE DMFSLFDESG SGEVDLRECV VALSVVCRPA RTLDTIQLAF KMYGAQEDGS VGEGDLSCIL KTALGVAELT VTDLFRAIDQ EEKGKITFAD FHRFAEMYPA FAEEYLYPDQ THFESCAETS PAPIPNGFCA DFSPENSDAG RKPVRKKLD -
Purification
- > 90% by SDS-PAGE
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Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) (AA 1-534) protein (His tag)
custom-made LPCAT1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) (AA 1-534) protein (His tag)custom-made LPCAT1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) protein (Myc-DYKDDDDK Tag)LPCAT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1M urea, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- LPCAT1 (Lysophosphatidylcholine Acyltransferase 1 (LPCAT1))
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Alternative Name
- LPCAT1
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Background
- Lysophosphatidylcholine acyltransferase 1, also known as LPCAT1, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Recombinant human LPCAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
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Molecular Weight
- 53.4 kDa (479aa)
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NCBI Accession
- NP_079106
Target
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