VEGF 165 (AA 1-191) protein (His tag)

Catalog No. ABIN6387506

Product Details

Target: VEGF 165

Protein Type: Recombinant

Protein Characteristics: AA 1-191

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRRHHHHH H

Purity: > 85 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate buffered saline (  pH 7.4), 30 % glycerol, 1  mM DTT, 0.1  mM PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: VEGF 165

Alternative Name: VEGF165

Background: Vascular endothelial growth factor A, also known as VEGF, is growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. This protein binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Recombinant human VEGF165 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.

Molecular Weight: 19.9 kDa (171aa)

NCBI Accession: NP_001165097

UniProt: P15692