NME2 Protein (AA 1-152)
Quick Overview for NME2 Protein (AA 1-152) (ABIN6387709)
Target
See all NME2 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-152
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Sequence
- MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
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Biological Activity Comment
- Specific activity is > 1,800unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
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Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)
custom-made NME2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)custom-made NME2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (GST tag)NME2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)NME2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)NME2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 1-152) proteinNME2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
Nucleoside diphosphate kinase B (NME2) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)NME2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- NME2 (Nucleoside diphosphate kinase B (NME2))
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Alternative Name
- NM23-H2/NME2
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Background
- NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 17.2 kDa (152aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001018149
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UniProt
- P22392
Target
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