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CST6 Protein (AA 29-149) (His tag)

Recombinant CST6 protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN6387720

Quick Overview for CST6 Protein (AA 29-149) (His tag) (ABIN6387720)

Target

See all CST6 Proteins
CST6 (Cystatin E/M (CST6))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 7
  • 1
Human

Source

  • 3
  • 2
  • 2
  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Purity

> 90 % by SDS - PAGE
  • Protein Characteristics

    AA 29-149

    Purification tag / Conjugate

    This CST6 protein is labelled with His tag.

    Sequence

    MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM

    Biological Activity Comment

    The IC50 value is < 10nM. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Bioactivity Validated

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Liquid. In 20  mM Tris-HCl buffer(  pH 8.0) containing 10 % glycerol, 1  mM DTT, 0.1M NaCl.

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target

    CST6 (Cystatin E/M (CST6))

    Alternative Name

    CST6

    Background

    CST6 is a member of the type 2 cystatin family. Some of the members are active cysteine protease inhibitors, while cystatin E/M moderate the inhibition of cathepsin B but is not active against cathepsin C. CST6 is secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and the response to systemic inflammation. Recombinant human CST6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

    Molecular Weight

    15.9 kDa (142aa), confirmed by MALDI-TOF

    NCBI Accession

    NP_001314

    UniProt

    Q15828
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