CST6 Protein (His tag)
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- Target See all CST6 Proteins
- CST6 (Cystatin E/M (CST6))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CST6 protein is labelled with His tag.
- Purpose
- Active Recombinant Human Cystatin-M/CST6 Protein
- Sequence
- RPQERMVGEL RDLSPDDPQV QKAAQAAVAS YNMGSNSIYY FRDTHIIKAQ SQLVAGIKYF LTMEMGSTDC RKTRVTGDHV DLTTCPLAAG AQQEKLRCDF EVLVVPWQNS SQLLKHNCVQ M
- Specificity
- Arg29-Met149
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.01 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is approximately 16 nM.
- Top Product
- Discover our top product CST6 Protein
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Cystatin E/M (CST6) (AA 61-149) protein (GST tag)
CST6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin E/M (CST6) (AA 29-149) protein (GST tag)CST6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin E/M (CST6) (AA 29-149) (Active) protein (His tag)CST6 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS Active
Cystatin E/M (CST6) protein (Myc-DYKDDDDK Tag)CST6 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Target
- CST6 (Cystatin E/M (CST6))
- Alternative Name
- Cystatin-M/CST6 (CST6 Products)
- Synonyms
- CST6 Protein, 1110017E11Rik Protein, N28197 Protein, ichq Protein, cystatin E/M Protein, CST6 Protein, Cst6 Protein
- Background
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Description: Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily. It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning. However, its protein was found only in skin and sweat glands by a third group. In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases. It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age. Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation.
Name: CST6
- Gene ID
- 1474
- UniProt
- Q15828
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