CST6 Protein (His tag)

Catalog No. ABIN7534344

This Recombinant CST6 protein is produced in HEK-293 Cells.

Quick Overview for CST6 Protein (His tag) (ABIN7534344)

Target: CST6 (Cystatin E/M (CST6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CST6 protein is labelled with His tag.

Purpose: Active Recombinant Human Cystatin-M/CST6 Protein

Sequence: RPQERMVGEL RDLSPDDPQV QKAAQAAVAS YNMGSNSIYY FRDTHIIKAQ SQLVAGIKYF LTMEMGSTDC RKTRVTGDHV DLTTCPLAAG AQQEKLRCDF EVLVVPWQNS SQLLKHNCVQ M

Specificity: Arg29-Met149

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.01 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is approximately 16 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: CST6 (Cystatin E/M (CST6))

Alternative Name: Cystatin-M/CST6

Background:

Description: Cystatin E/M encoded by the CST6 gene is a member of family 2 of the cystatin superfamily. It inhibits papain and cathepsin B, two of the cysteine proteases. Its mRNA was found in many tissues by the two groups who did initial cloning. However, its protein was found only in skin and sweat glands by a third group. In addition to being a cysteine protease inhibitor, cystatin E/M is also a substrate for transglutaminases. It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice, with a null mutation in the cystatin E/M gene, have defects in epidermal cornification and die between 5 and 12 days of age. Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation.

Name: CST6

Gene ID: 1474

UniProt: Q15828