Endoglin Protein (ENG) (AA 27-581) (His tag)
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- Target See all Endoglin (ENG) Proteins
- Endoglin (ENG)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 27-581
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Endoglin protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Activity Assay (AcA)
- Sequence
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ERVGCDLQPV DPTRGEVTFT TSQVSEGCVA QAANAVREVH VLFLDFPGML SHLELTLQAS KQNGTETQEV FLVLVSNKNV FVKFQAPEIP LHLAYDSSLV IFQGQPRVNI TVLPSLTSRK QILDWAATKG AITSIAALDD PQSIVLQLGQ DPKAPFLCLP EAHKDMGATL EWQPRAQTPV QSCRLEGVSG HKEAYILRIL PGSEAGPRTV TVMMELSCTS GDAILILHGP PYVSWFIDIN HSMQILTTGE YSVKIFPGSK VKGVELPDTP QGLIAEARKL NASIVTSFVE LPLVSNVSLR ASSCGGVFQT TPAPVVTTPP KDTCSPVLLM SLIQPKCGNQ VMTLALNKKH VQTLQCTITG LTFWDSSCQA EDTDDHLVLS SAYSSCGMKV TAHVVSNEVI ISFPSGSPPL RKKVQCIDMD SLSFQLGLYL SPHFLQASNT IELGQQAFVQ VSVSPLTSEV TVQLDSCHLD LGPEGDMVEL IQSRTAKGSC VTLLSPSPEG DPRFSFLLRV YMVPTPTAGT LSCNLALRPS TLSQEVYKTV SMRLNIVSPD LSGKG
- Purity
- > 95% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA with Human TGF-beta RI (CAT# ATGP4134).
- Top Product
- Discover our top product ENG Protein
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Endoglin (ENG) (AA 26-586) protein (His-Avi Tag)
ENG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Endoglin (ENG) (AA 1-658) protein (GST tag)ENG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 394-581) protein (His-IF2DI Tag)ENG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Endoglin (ENG) (AA 27-658) protein (GST tag)ENG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 26-586) protein (His tag)ENG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85% by SDS-PAGE SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- Endoglin (ENG)
- Alternative Name
- Endoglin/CD105 (ENG Products)
- Background
- Eng, also known as endoglin, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. It is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. Recombinant mouse Eng, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 60.9 kDa (563aa)
- NCBI Accession
- NP_031958
- UniProt
- Q63961
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