GDA Protein (AA 1-454)
Quick Overview for GDA Protein (AA 1-454) (ABIN5854639)
Target
See all GDA ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-454
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Sequence
- MCAAQMPPLA HIFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEEASQ QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHASQYSF AGSSIDLPLL EWLTKYTFPA EHRFQNIDFA EEVYTRVVRR TLKNGTTTAC YFATIHTDSS LLLADITDKF GQRAFVGKVC MDLNDTFPEY KETTEESIKE TERFVSEMLQ KNYSRVKPIV TPRFSLSCSE TLMGELGNIA KTRDLHIQSH ISENRDEVEA VKNLYPSYKN YTSVYDKNNL LTNKTVMAHG CYLSAEELNV FHERGASIAH CPNSNLSLSS GFLNVLEVLK HEVKIGLGTD VAGGYSYSML DAIRRAVMVS NILLINKVNE KSLTLKEVFR LATLGGSQAL GLDGEIGNFE VGKEFDAILI NPKASDSPID LFYGDFFGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF SSSV
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Biological Activity Comment
- Specific activity is > 2,000pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
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Guanine Deaminase (GDA) (AA 1-454) protein (His tag)
custom-made GDA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Guanine Deaminase (GDA) (AA 1-454) protein (His tag)custom-made GDA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Guanine Deaminase (GDA) (AA 1-454) protein (GST tag)GDA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Guanine Deaminase (GDA) (AA 1-454) (Active) protein (His tag)GDA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, EAA Active
Guanine Deaminase (GDA) (AA 1-454) protein (His-IF2DI Tag)GDA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Guanine Deaminase (GDA) (AA 1-454) protein (His-IF2DI Tag)GDA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Guanine Deaminase (GDA) proteinGDA Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- GDA (Guanine Deaminase (GDA))
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Alternative Name
- Guanine deaminase/GDA
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Background
- GDA, also known as guanine deaminase, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA, was expressed in E. coli and purified by conventional chromatography techniques.
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Molecular Weight
- 51 kDa (454aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_004284
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UniProt
- Q9Y2T3
Target
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