GAPDH Protein (AA 1-335)
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- Target See all GAPDH Proteins
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-335
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- Sequence
- MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
- Purity
- > 95% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 50unit/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
- Top Product
- Discover our top product GAPDH Protein
-
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-333) protein (His tag)
GAPDH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-335) protein (His tag)GAPDH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) protein (GST tag)GAPDH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) protein (GST tag)GAPDH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) proteinGAPDH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Alternative Name
- GAPDH (GAPDH Products)
- Background
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36- kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1, 3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36 kDa (335aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_002037
- UniProt
- P04406
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