BTD Protein (AA 44-545) (His tag)
Quick Overview for BTD Protein (AA 44-545) (His tag) (ABIN6387911)
Target
See all BTD ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 44-545
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Purification tag / Conjugate
- This BTD protein is labelled with His tag.
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Sequence
- AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biotinidase (BTD) (AA 1-543) protein (GST tag)
BTD Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Biotinidase (BTD) protein (Myc-DYKDDDDK Tag)BTD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- BTD (Biotinidase (BTD))
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Alternative Name
- Biotinidase/BTD
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Background
- BTD, also known as biotinidase isoform 1, is a member of the nitrilase superfamily, which consists of 12 families of nitrilases, amidases, carbamylases, and N-acyltrasferases. It catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. It may have an important regulatory role in chromatin/DNA function. Recombinant human BTD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 57.8 kDa (510aa)
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NCBI Accession
- NP_001268652
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UniProt
- P43251
Target
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