BTD Protein (His tag)
Quick Overview for BTD Protein (His tag) (ABIN7194393)
Target
See all BTD ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This BTD protein is labelled with His tag.
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Purpose
- Recombinant Human Biotinidase/BTD Protein (His Tag)
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Sequence
- Met 1-Asp543
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Characteristics
- A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is > 500pmol/min/μg.
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Biotinidase (BTD) (AA 44-545) protein (His tag)
BTD Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % SDS
Biotinidase (BTD) (AA 1-543) protein (GST tag)BTD Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Biotinidase (BTD) protein (Myc-DYKDDDDK Tag)BTD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- BTD (Biotinidase (BTD))
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Alternative Name
- Biotinidase/BTD
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Background
- BTD,Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing offats,carbohydrates, andproteins. Biotin is attached to these carboxylase enzymes through anamino acid(the building material of proteins) calledlysine, forming a complex calledbiocytin.
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Molecular Weight
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Calculated MW: 58.2 kDa
Observed MW: 66-76 kDa
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Gene ID
- 686
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UniProt
- P43251
Target
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