BTD Protein (His tag)
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- Target See all BTD Proteins
- BTD (Biotinidase (BTD))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BTD protein is labelled with His tag.
- Purpose
- Recombinant Human Biotinidase/BTD Protein (His Tag)(Active)
- Sequence
- Met 1-Asp543
- Characteristics
- A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmol/min/μg.
- Top Product
- Discover our top product BTD Protein
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Biotinidase (BTD) (AA 1-543) protein (GST tag)
BTD Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Biotinidase (BTD) protein (Myc-DYKDDDDK Tag)BTD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Biotinidase (BTD) (AA 44-545) protein (His tag)BTD Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- BTD (Biotinidase (BTD))
- Alternative Name
- Biotinidase/BTD (BTD Products)
- Synonyms
- BTD Protein, CG3599 Protein, CT12113 Protein, Dmel\\CG3599 Protein, zgc:153333 Protein, Biotinase Protein, Biotinidase Protein, biotinidase Protein, Btnd Protein, btd Protein, BTD Protein, Btd Protein
- Background
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Background: Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.
Synonym: BTD
- Molecular Weight
- 58.2 kDa
- UniProt
- P43251
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