ECHS1 Protein (AA 28-290) (His tag)

Catalog No. ABIN6388011

This Recombinant ECHS1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for ECHS1 Protein (AA 28-290) (His tag) (ABIN6388011)

Target: ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95 %

Product Details

Protein Characteristics: AA 28-290

Purification tag / Conjugate: This ECHS1 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

Purification: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 150unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol, 100 mM NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))

Alternative Name: ECHS1

Background: Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 30.6 kDa (284aa) confirmed by MALDI-TOF

Pathways: Monocarboxylic Acid Catabolic Process