GFRA1 Protein (AA 25-430) (hIgG-His-tag)
Quick Overview for GFRA1 Protein (AA 25-430) (hIgG-His-tag) (ABIN6388021)
Target
See all GFRA1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 25-430
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Purification tag / Conjugate
- This GFRA1 protein is labelled with hIgG-His-tag.
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Sequence
- DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLTS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRA VPFISDVFQQ VEHISKGNNC LDAAKACNLD DTCKKYRSAY ITPCTTSMSN EVCNRRKCHK ALRQFFDKVP AKHSYGMLFC SCRDIACTER RRQTIVPVCS YEERERPNCL SLQDSCKTNY ICRSRLADFF TNCQPESRSV SNCLKENYAD CLLAYSGLIG TVMTPNYVDS SSLSVAPWCD CSNSGNDLED CLKFLNFFKD NTCLKNAIQA FGNGSDVTMW QPAPPVQTTT ATTTTAFRVK NKPLGPAGSE NEIPTHVLPP CANLQAQKLK SNVSGSTHLC LSDSDFGKDG LAGASSLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
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Purification
- > 85% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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GDNF Family Receptor alpha 1 (GFRA1) (AA 25-424) (Active) protein (His tag)
GFRA1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
GDNF Family Receptor alpha 1 (GFRA1) (AA 1-460) protein (GST tag)GFRA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (Fc Tag)GFRA1 Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
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Alternative Name
- GDNFR alpha-1/GFRA1
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Background
- Gfra1, also known as GDNF family receptor alpha-1, is a member of the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI) -linked cell surface receptor for both Glial cell line-derived growth factor (GDNF), neurturin (NTN), and mediates activation of the RET tyrosine kinase receptor. This protein is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Recombinant rat Gfra1 protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 72.3 kDa (645aa)
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NCBI Accession
- NP_037091
Target
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