DPH2 Protein (AA 1-489) (His tag)
Quick Overview for DPH2 Protein (AA 1-489) (His tag) (ABIN6388024)
Target
See all DPH2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-489
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Purification tag / Conjugate
- This DPH2 protein is labelled with His tag.
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Sequence
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MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSG
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Purification
- > 85% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489) protein (His tag)
custom-made DPH2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489) protein (His tag)custom-made DPH2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489) protein (GST tag)DPH2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489), (full length) protein (His-SUMO Tag)DPH2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Diphthamide Biosynthesis Protein 2 (DPH2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DPH2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 1 mM DTT, 20 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))
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Alternative Name
- DPH2
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Background
- DPH2, also known as diphthamide biosynthesis protein 2, is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. It is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE). It was identified by its ability to complement a diphthamide mutant strain, and thus functions in diphthamide biosynthesis. Its loss pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Recombinant human DPH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 53.1 kDa (497aa)
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NCBI Accession
- NP_001375
Target
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