KIT Ligand Protein (KITLG) (AA 26-189)
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- Target See all KIT Ligand (KITLG) Proteins
- KIT Ligand (KITLG)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 26-189
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS), Activity Assay (AcA)
- Sequence
- MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
- Purity
- > 95% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤10ng/ml.
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KIT Ligand (KITLG) (Active) protein
KITLG Origin: Human Host: Yeast (Pichia pastoris) > 95 % by SDS-PAGE Active
KIT Ligand (KITLG) (AA 26-214) protein (Fc Tag)KITLG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-190) protein (His tag)KITLG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His tag)KITLG Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His-IF2DI Tag)KITLG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
KIT Ligand (KITLG) (AA 1-245) protein (GST tag)KITLG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Rat Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) proteinKITLG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate buffer saline ( pH 7.4)
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- KIT Ligand (KITLG)
- Alternative Name
- c-kit Ligand/SCF (KITLG Products)
- Background
- Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- Molecular Weight
- 18.5 kDa (165aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_000890
- UniProt
- P21583
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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