ALDOC Protein (AA 1-364)

Catalog No. ABIN5855049

This Recombinant ALDOC protein is expressed in Escherichia coli (E. coli).

Quick Overview for ALDOC Protein (AA 1-364) (ABIN5855049)

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-364

Sequence: MPHSYPALSA EQKKELSDIA LRIVAPGKGI LAADESVGSM AKRLSQIGVE NTEENRRLYR QVLFSADDRV KKCIGGVIFF HETLYQKDDN GVPFVRTIQD KGIVVGIKVD KGVVPLAGTD GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKISERTPS ALAILENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC PIKYTPEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SFNLNAINRC PLPRPWALTF SYGRALQASA LNAWRGQRDN AGAATEEFIK RAEVNGLAAQ GKYEGSGEDG GAAAQSLYIA NHAY

Biological Activity Comment: Specific activity is > 6unit/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3- phosphate per minute at pH 7.5 at 37C

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl (  pH 8.0) containing 20 % Glycerol, 2  mM DTT, 0.1M NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Alternative Name: ALDOC

Background: ALDOC, also as known as fructose bisphosphate C, is a member of the class 1 fructose-biphosphate aldolase family. This protein is a ubiquitous enzyme that catalyzes the reversible aldol cleavage of fructose-bisphosphate and fructose 1-phospate to digydroxyacetone phosphate and either glyceral-dehyde-3-phosphate or glyceraldegyde, respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain. Recombinant human ALDOC was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 39.4 kDa (364aa) confirmed by MALDI-TOF

NCBI Accession: NP_005156

UniProt: P09972