UGDH Protein (AA 1-493) (His tag)
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- Target See all UGDH Proteins
- UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-493
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UGDH protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMVEIKKI CCIGAGYVGG PTCSVIAHMC PEIRVTVVDV NEARINAWNS PTLPIYEPGL KEVVESCRGK NLFFSTNIDD AIREADLVFI SVNTPTKTYG MGKGRAADLK YIEACARRIV QNSNGYKIVT EKSTVPVRAA ESIRRIFDAN TKPNLNLQVL SNPEFLAEGT AIKDLKNPDR VLIGGDETPE GQKAVRALCA VYEHWVPKEK ILTTNTWSSE LSKLAANAFL AQRISSINSI SALCEATGAD VEEVATAIGM DQRIGNKFLK ASVGFGGSCF QKDVLNLVYL CEALNLPEVA RYWQQVIDMN DYQRRRFASR IIDSLFNTVT DKKIAILGFA FKKDTGDTRE SSSIYISKYL MDEGAHLHIY DPKVPREQIV VDLSHPGVSA DDQVSRLVTI SKDPYEACDG AHALVICTEW DMFKELDYER IHKKMLKPAF IFDGRRVLDG LHSELQTIGF QIETIGKKVS SKRIPYTPGE IPKFSLQDPP NKKPKV
- Purity
- > 90% by SDS-PAGE
- Biological Activity Comment
- Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C
- Top Product
- Discover our top product UGDH Protein
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UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494) protein (His tag)
custom-made UGDH Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494) (Active) protein (His tag)UGDH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, EAA Active
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494) protein (GST tag)UGDH Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 395-494) protein (GST tag)UGDH Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-493) protein (His tag)UGDH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
UDP-Glucose 6-Dehydrogenase (UGDH) (AA 1-494), (full length) protein (His-SUMO Tag)UGDH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
UDP-Glucose 6-Dehydrogenase (UGDH) protein (Myc-DYKDDDDK Tag)UGDH Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM MES buffer ( pH 5.0) containing 20 % glycerol, 150 mM NaCl, 1 mM EDTA
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))
- Alternative Name
- UDP-glucose dehydrogenase/UGDH (UGDH Products)
- Background
- Ugdh, also known as UDP-glucose 6-dehydrohenase, is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant mouse Ugdh, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 57.2 kDa (516aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_033492
- UniProt
- O70475
- Pathways
- Glycosaminoglycan Metabolic Process
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