UGDH Protein (AA 1-494) (His tag)

Catalog No. ABIN667140

This Recombinant UGDH protein is expressed in Escherichia coli (E. coli).

Quick Overview for UGDH Protein (AA 1-494) (His tag) (ABIN667140)

Target: UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-494

Purification tag / Conjugate: This UGDH protein is labelled with His tag.

Sequence: M FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV

Characteristics: UGDH, 1-494aa, Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 2500pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT, 0.1M NaCl, and 1 mM EDTA

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))

Alternative Name: UDP-glucose dehydrogenase/UGDH

Background: UGDH is a member of the uDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts uDP-glucose to uDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human uGDH protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Molecular Weight: 59.5 kDa (533aa) confirmed by MALDI-TOF

NCBI Accession: NP_003350

Pathways: Glycosaminoglycan Metabolic Process