CRMP1 Protein (AA 1-572) (His tag)
Quick Overview for CRMP1 Protein (AA 1-572) (His tag) (ABIN6388174)
Target
See all CRMP1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-572
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Purification tag / Conjugate
- This CRMP1 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSEFMSHQG KKSIPHITSD RLLIRGGRII NDDQSFYADV YLEDGLIKQI GENLIVPGGV KTIEANGRMV IPGGIDVNTY LQKPSQGMTS ADDFFQGTKA ALAGGTTMII DHVVPEPGSS LLTSFEKWHE AADTKSCCDY SLHVDITSWY DGVREELEVL VQDKGVNSFQ VYMAYKDLYQ MSDSQLYEAF TFLKGLGAVI LVHAENGDLI AQEQKRILEM GITGPEGHAL SRPEELEAEA VFRAIAIAGR INCPVYITKV MSKSAADIIA LARKKGPLVF GEPIAASLGT DGTHYWSKNW AKAAAFVTSP PLSPDPTTPD YLTSLLACGD LQVTGSGHCP YSTAQKAVGK DNFTLIPEGV NGIEERMTVV WDKAVATGKM DENQFVAVTS TNAAKIFNLY PRKGRIAVGS DADVVIWDPD KMKTITAKSH KSTVEYNIFE GMECHGSPLV VISQGKIVFE DGNISVSKGM GRFIPRKPFP EHLYQRVRIR SKVFGLHSVS RGMYDGPVYE VPATPKHAAP APSAKSSPSK HQPPPIRNLH QSNFSLSGAQ IDDNNPRRTG HRIVAPPGGR SNITSLG
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Purification
- > 90% by SDS-PAGE
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Collapsin Response Mediator Protein 1 (CRMP1) (AA 1-572) protein (His tag)
custom-made CRMP1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Collapsin Response Mediator Protein 1 (CRMP1) (AA 1-572) protein (His tag)custom-made CRMP1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Collapsin Response Mediator Protein 1 (CRMP1) (AA 1-566) protein (GST tag)CRMP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Collapsin Response Mediator Protein 1 (CRMP1) (AA 1-572) protein (His-IF2DI Tag)CRMP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Collapsin Response Mediator Protein 1 (CRMP1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CRMP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Collapsin Response Mediator Protein 1 (CRMP1) (AA 1-572), (full length) protein (His-SUMO Tag)CRMP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.5) containing 40 % glycerol, 0.2M NaCl, 0.1 mM PMSF
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CRMP1 (Collapsin Response Mediator Protein 1 (CRMP1))
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Alternative Name
- CRMP1
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Background
- Crmp1, also known as Collapsin response mediator protein 1, is necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. It plays a role in axon guidance, invasive growth and cell migration and may participate in cytokinesis. Recombinant mouse Crmp1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 64.8 kDa (597aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_031791
Target
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