EIF5A Protein (AA 1-154)

Catalog No. ABIN666636

This Recombinant EIF5A protein is expressed in Escherichia coli (E. coli).

Quick Overview for EIF5A Protein (AA 1-154) (ABIN666636)

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-154

Sequence: MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK

Characteristics: eIF5A, 1-154 aa, Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 mM Tris-HCl buffer ( pH 7.5) containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Alternative Name: EIF5A

Background: Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation, however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer. Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 16.8 kDa (154aa) confirmed by MALDI-TOF

NCBI Accession: NP_001961

Pathways: Regulation of Muscle Cell Differentiation