His-Streptavidin (AA 25-183) protein (His tag)

Catalog No. ABIN666648

Product Details

Target: His-Streptavidin

Protein Type: Recombinant

Protein Characteristics: AA 25-183

Origin: Streptomyces avidinii

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Characteristics: His-Streptavidin, 25-183aa, Streptomyces avidinii, His tagged, Recombinant, E.coli

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid in 20mM Tris pH 7.5

Storage: 4 °C

References

Lanz, Masi, Porciello, Cohnen, Cipria, Prakaash, Bálint, Raggiaschi, Galgano, Cole, Lepore, Dushek, Dustin, Sansom, Kalli, Acuto: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." in: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).

Target Details

Target: His-Streptavidin

Background: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2. NCBI no.: CAA27265, P22629

Molecular Weight: 17 kDa (167 aa), confirmed by MALDI-TOF.