Streptavidin Protein (AA 25-183) (His tag)
(1 reference)Quick Overview for Streptavidin Protein (AA 25-183) (His tag) (ABIN666648)
Target
See all Streptavidin ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 25-183
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Purification tag / Conjugate
- This Streptavidin protein is labelled with His tag.
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Sequence
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DP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ -
Characteristics
- His-Streptavidin, 25-183aa, Streptomyces avidinii, His tagged, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Streptavidin protein (HRP)
Origin: Streptomyces avidinii Host: Streptomyces avidinii Native WB, ELISA, FM, IHC, DB, IF, IEM
Streptavidin protein (PE)Origin: Streptomyces avidinii Host: Streptomyces avidinii Native ELISA, FM, FACS, MA, DB, IF, BCA, Func
Streptavidin (AA 37-159) protein (His tag)Origin: Streptomyces Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Streptavidin protein (TRITC)Origin: Streptomyces avidinii Host: Streptomyces avidinii Native FM, FLISA, IHC, FISH
Streptavidin protein (Atto 425)Origin: Streptomyces avidinii Host: Streptomyces avidinii Native WB, FM, FLISA, DB, IF
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 7.5)
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." in: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).
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: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." in: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).
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- Streptavidin
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Background
- Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 17.5 kDa (167aa) confirmed by MALDI-TOF
Target
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