STIM1 Protein (AA 23-213) (Calmodulin (CaM))

Catalog No. ABIN666653

This Recombinant STIM1 protein is produced in Escherichia coli (E. coli).

Quick Overview for STIM1 Protein (AA 23-213) (Calmodulin (CaM)) (ABIN666653)

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 23-213

Purification tag / Conjugate: This STIM1 protein is labelled with Calmodulin (CaM).

Sequence: MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMLSHSHSEK ATGTSSGANS EESTAAEFCR IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVVQW LITYVELPQY EETFRKLQLS GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKD

Characteristics: STIM1, 23-213aa, Human, CaM tagged, Recombinant, E.coli

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5)

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1

Background: Stromal interaction molecule 1 (STIM1) is a transmembrane protein that is essential for store-operated Ca (2+) entry, a process of extracellular Ca (2+) influx in response to the depletion of Ca (2+) stores in the endoplasmic reticulum (ER). STIM1 localizes predominantly to the ER, upon Ca (2+) release from the ER, STIM1 translocates to the ER-plasma membrane junctions and activates Ca (2+) channels. Recombinant Calmodulin tagged STIM was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 38 kDa (343aa) confirmed by MALDI-TOF

NCBI Accession: NP_003147

Pathways: TCR Signaling, BCR Signaling