STIM1 Protein (His tag)
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- Target See all STIM1 Proteins
- STIM1 (Stromal Interaction Molecule 1 (STIM1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This STIM1 protein is labelled with His tag.
- Purpose
- Recombinant Human STIM1/GOK Protein (His Tag)
- Sequence
- Met 1-Asp 213
- Characteristics
- A DNA sequence encoding the human STIM1 (NP_003147.2) extracellular domain (Met 1-Asp 213) was expressed, with a polyhistidine tag at the C-terminus.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product STIM1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- STIM1 (Stromal Interaction Molecule 1 (STIM1))
- Alternative Name
- STIM1/GOK (STIM1 Products)
- Synonyms
- stim1 Protein, MGC76045 Protein, STIM1 Protein, si:dkey-24p1.5 Protein, MGC154748 Protein, si:ch211-112l17.2 Protein, Stim1 Protein, D11S4896E Protein, GOK Protein, TAM Protein, SIM Protein, stromal interaction molecule 1 Protein, stromal interaction molecule 1a Protein, stromal interaction molecule 1 L homeolog Protein, stromal interaction molecule 1b Protein, STIM1 Protein, stim1 Protein, stim1a Protein, stim1.L Protein, stim1b Protein, Stim1 Protein
- Background
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Background: Stromal interaction molecule 1, also known as STIM1 and GOK, is a cell membrane, a single-pass type I membrane protein and a endoplasmic reticulum membrane protein. STIM1 / GOK is ubiquitously expressed in various human primary cells and tumor cell lines. It contains one EF-hand domain and one SAM (sterile alpha motif) domain. STIM1 / GOK plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. It acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, STIM1 / GOK translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A / ORAI1. Transfection of STIM1 / GOK into cells derived from a rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable levels of STIM1 can induce cell death, suggesting a possible role in the control of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.
Synonym: D11S4896E;GOK;IMD10;STRMK;TAM;TAM1
- Molecular Weight
- 23.3 kDa
- NCBI Accession
- NP_003147
- Pathways
- TCR Signaling, BCR Signaling
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