PPIB Protein (AA 26-216)
Quick Overview for PPIB Protein (AA 26-216) (ABIN666694)
Target
See all PPIB ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 26-216
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Sequence
- MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
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Characteristics
- Cyclophilin B, 26- 216aa , Human, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 1,500nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
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Cyclophilin B (PPIB) (AA 34-216) protein (GST tag)
PPIB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cyclophilin B (PPIB) (AA 34-216) protein (His tag)PPIB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cyclophilin B (PPIB) (AA 1-216) protein (GST tag)PPIB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Cyclophilin B (PPIB) (AA 34-216) (Active) protein (His tag)PPIB Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 mM NaCl, 0.5 mM DTT, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PPIB (Cyclophilin B (PPIB))
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Alternative Name
- Cyclophilin B/PPIB
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Background
- Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques
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Molecular Weight
- 21.2 kDa (192aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000933
Target
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