PPIB Protein (AA 34-216) (His tag)
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- Target See all PPIB Proteins
- PPIB (Cyclophilin B (PPIB))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 34-216
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPIB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMNDKKKG PKVTVKVYFD LQIGDESVGR VVFGLFGKTV PKTVDNFVAL ATGEKGFGYK NSKFHRVIKD FMIQGGDFTR GDGTGGKSIY GERFPDENFK LKHYGPGWVS MANAGKDTNG SQFFITTVKT SWLDGKHVVF GKVLEGMDVV RKVESTKTDS RDKPLKDVII VDSGKIEVEK PFAIAKE
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 1,000 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
- Top Product
- Discover our top product PPIB Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PPIB (Cyclophilin B (PPIB))
- Alternative Name
- Ppib (PPIB Products)
- Synonyms
- cypb Protein, scylp Protein, cyp-s1 Protein, PPIB Protein, Ppib Protein, ACYPI004891 Protein, AA408962 Protein, AA553318 Protein, AI844835 Protein, Cphn-2 Protein, Cphn2 Protein, CyP-20b Protein, CypB Protein, Scylp Protein, CYP-S1 Protein, CYPB Protein, OI9 Protein, SCYLP Protein, cb87 Protein, sb:cb87 Protein, wu:fa97f08 Protein, zgc:73214 Protein, zgc:86796 Protein, peptidylprolyl isomerase B Protein, peptidylprolyl isomerase B (cyclophilin B) Protein, peptidylprolyl isomerase B L homeolog Protein, ppib Protein, PPIB Protein, Ppib Protein, CC1G_03223 Protein, ppib.L Protein
- Background
- Ppib, also known as peptidyl-prolyl cis-trans isomerase B, is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A -mediated immunosuppression. Recombinant mouse Ppib, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 22.7 kDa (207aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_035279
- UniProt
- P24369
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