Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1) (AA 1-316) (Active) Protein

Catalog No. ABIN666726

This Recombinant Aldo-Keto Reductase Family 1 Member B10-Like 1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1) (AA 1-316) (Active) Protein (ABIN666726)

Target: Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Enzyme Activity Assay (EAA), SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-316

Sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF

Characteristics: Aldose reductase, 1-316aa, Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is >800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1)

Alternative Name: Aldo-keto reductase 1B1/AKR1B1

Background: Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 35.8 kDa (316aa) confirmed by MALDI-TOF

NCBI Accession: NP_001619