CITED2 Protein (AA 1-270) (His tag)
Quick Overview for CITED2 Protein (AA 1-270) (His tag) (ABIN666771)
Target
See all CITED2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-270
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Purification tag / Conjugate
- This CITED2 protein is labelled with His tag.
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Sequence
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MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG PPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP AAGHQMNGTN QHFRDCNPKH SGGSSTPGGS GGSSTPGGSG SSSGGGAGSS NSGGGSGSGN MPASVAHVPA AMLPPNVIDT DFIDEEVLMS LVIEMGLDRI KELPELWLGQ NEFDFMTDFV CKQQPSRVSC
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Characteristics
- CITED2, 1- 270 aa, Human, His-tagged, Recombinant, E.coli
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (AA 1-269) protein (His tag)
custom-made CITED2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (AA 1-270) protein (His tag)custom-made CITED2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (AA 1-270) protein (GST tag)CITED2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CITED2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 50 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))
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Alternative Name
- CITED2
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Background
- CITED2 is an important transcriptional cofactor involved in multiple organ development. It is a cAMP-responsive element-binding protein (CBP) /p300 interacting transcriptional modulator and a proposed negative regulator for hypoxia-inducible factor (HIF) -1alpha through its competitive binding with HIF-1alpha to CBP/p300. CITED2 is necessary for mouse fetal liver hematopoiesis and is required for the proper formation of the hyaloid vasculature and for lens morphogenesis. Recombinant CITED2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 29.5 kDa (278aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_006070
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Pathways
- Tube Formation
Target
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