CCL3 Protein (AA 24-92) (His tag)
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- Target See all CCL3 Proteins
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-92
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CCL3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
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SLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA - Characteristics
- CCL3, 24-92aa, Human, His tag, E.coli
- Purity
- > 90% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product CCL3 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 10mM Sodium Citrate ( pH3.5) containing 10% glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
- Alternative Name
- CCL3/MIP-1 alpha (CCL3 Products)
- Background
- CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner. Recombinant human CCL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- Molecular Weight
- 10 kDa (90aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
- NCBI Accession
- NP_002974
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Autophagy
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