VEGF 165 (AA 207-371) protein (His tag)

Catalog No. ABIN666798

Product Details

Target: VEGF 165

Protein Type: Recombinant

Protein Characteristics: AA 207-371

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Sequence: APMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

Characteristics: VEGF165, 207-371aa, Human, His tag, E.coli

Purity: > 90% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20mM Tris-HCl(pH8.5), 50%glycerol, 5mM DTT, 200mM NaCl, 2mM EDTA

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: VEGF 165

Background: Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molecular Weight: 21.4 kDa (186aa) conformed by MALDI-TOF

NCBI Accession: NP_001020539