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DnaK (AA 1-638), (full length) Protein

This Recombinant protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN666804

Quick Overview for DnaK (AA 1-638), (full length) Protein (ABIN666804)

Target

DnaK

Protein Type

Recombinant

Origin

  • 4
  • 2
  • 1
E. coli

Source

  • 7
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Purity

> 95 % by SDS - PAGE
  • Protein Characteristics

    full length, AA 1-638

    Characteristics

    Dnak(full length, 1-638) E.coli, Recombinant, E.coli
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  • Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/ml (determined by Bradford assay)

    Buffer

    Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5mM DTT,10%Glycerol

    Storage

    4 °C
  • Target

    DnaK

    Background

    DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (amino acids 1-638) was amplified by PCR and cloned into an E. coli expression vector. DnaK 1-638 was overexpressed in E. coli and was purified to apparent homogeneity by using conventional column chromatography techniques. Synonyms: Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,. NCBI no.: NP_414555

    Molecular Weight

    69kDa (638aa)
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