DnaK (AA 1-638), (full length) Protein

Catalog No. ABIN666804

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for DnaK (AA 1-638), (full length) Protein (ABIN666804)

Target: DnaK

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: full length, AA 1-638

Characteristics: Dnak(full length, 1-638) E.coli, Recombinant, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5mM DTT,10%Glycerol

Storage: 4 °C

Target Details

Target: DnaK

Background: DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (amino acids 1-638) was amplified by PCR and cloned into an E. coli expression vector. DnaK 1-638 was overexpressed in E. coli and was purified to apparent homogeneity by using conventional column chromatography techniques. Synonyms: Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,. NCBI no.: NP_414555

Molecular Weight: 69kDa (638aa)