DnaK Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and RP- HPLC
WB
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- Target
- DnaK
- Protein Type
- Recombinant
- Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Application
- Western Blotting (WB)
- Purpose
- Recombinant Human DnaKHsp70) produced in E. coli is a single, non- glycosylated polypeptide chain containing 661 amino acids fused to His-tag at the N-terminus with a total molecular weight of 72.2kDa.
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGD TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D
- Characteristics
- Escherichia coli
- Purity
- > 95 % as determined by SDS-PAGE and RP- HPLC
-
DnaK (AA 1-638), (full length) protein
Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Reconstitution
- Dilute in PBS or medium that is identical to that used in the assay system.
- Concentration
- Lot specific
- Buffer
- Sterile-filtered colorless solution contains 20 mM Tris, pH 7.5, and 2 mM DTT having a protein concentration of 1 mg/mL.
- Storage
- 4 °C,-20 °C
-
- Target
- DnaK
- Background
- Hsp70 plays an important role in protein folding and in protecting cells from stress. In most species there are many proteins that belong to the Hsp70 family. Some are expressed only under stress conditions while others are present in cells under normal conditions and are not heat-inducible.,Hsp70
- Molecular Weight
- 72.2 kDa
- UniProt
- P08107
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