ADIPOQ Protein (AA 111-247)
Quick Overview for ADIPOQ Protein (AA 111-247) (ABIN666808)
Target
See all ADIPOQ ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 111-247
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Sequence
- MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN
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Characteristics
- Adiponectin(111-247aa, globular domain), Mouse, Recombinant, expressed in E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Adiponectin (ADIPOQ) (Active) protein
ADIPOQ Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Adiponectin (ADIPOQ) (AA 18-247) protein (Fc Tag)ADIPOQ Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Adiponectin (ADIPOQ) protein (His tag)ADIPOQ Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Adiponectin (ADIPOQ) protein (His tag)ADIPOQ Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Adiponectin (ADIPOQ) (AA 19-244) protein (Fc Tag)ADIPOQ Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Adiponectin (ADIPOQ) (AA 111-247) protein (His-IF2DI Tag)ADIPOQ Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Adiponectin (ADIPOQ) (AA 19-244) protein (His tag)ADIPOQ Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Adiponectin (ADIPOQ) (AA 18-247) protein (His-IF2DI Tag)ADIPOQ Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Adiponectin (ADIPOQ) (AA 111-210) protein (GST tag)ADIPOQ Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 7.5) containing 50 mM NaCl, 5 mM DTT, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ADIPOQ (Adiponectin (ADIPOQ))
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Alternative Name
- Adiponectin
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Background
- Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30 kDa and exclusively expressed in differentiated adipocytes. Adiponectin (Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain (gAcrp30). Adiponectin (Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 (247aa) or gAcrp30 (globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30 (amino acid residues, 111-247, gAcrp30) was overexpressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 16 kDa (138aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_033735
Target
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