GAGA-POZ (AA 1-130) Protein

Catalog No. ABIN666837

This Recombinant GAGA-POZ protein is expressed in Escherichia coli (E. coli).

Quick Overview for GAGA-POZ (AA 1-130) Protein (ABIN666837)

Target: GAGA-POZ

Protein Type: Recombinant

Origin: Drosophila melanogaster

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-130

Sequence: MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL DLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQ TVTKDDYTTH

Characteristics: GAGA-POZ domain(1-130aa), Drosophila melanogaster Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM HEPES buffer ( pH 7.4) containing 25 mM NaCl

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GAGA-POZ

Background: The GAGA factor is a sequence-specific DNA-binding protein, which participates in the regulation of the expression of a variety of different classes of genes in Drosophila such as many developmentally regulated genes, stress induced genes, and cell cycle regulated genes, as well as housekeeping genes. GAGA contains a C-terminal glutamine-rich domain and a highly conserved N-terminal POZ domain which reported to be involved in self-oligomerization in a number of other POZ domain containing proteins. In case of GAGA protein, the N-terminal POZ domain mediates the formation of oligomers both in vitro and in vivo. GAGA-POZ domain was overexpressed in E. coli and purified by using conventional chromatography techniques

Molecular Weight: 14 kDa (130aa) confirmed by MALDI-TOF

NCBI Accession: NP_996080