RPS27A Protein (AA 1-76)
Quick Overview for RPS27A Protein (AA 1-76) (ABIN666840)
Target
See all RPS27A ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-76
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Sequence
- MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
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Characteristics
- Ubiquitin Human, Recombinant, expressed in E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 50 mM HEPES buffer ( pH 7.4) containing 150 mM NaCl, 1 mM DTT, 10 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- RPS27A (Ribosomal Protein S27a (RPS27A))
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Alternative Name
- Ubiquitin/RPS27A
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Background
- Ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity. ubiquitin (76amino acid) was overexpressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 8.6 kDa (76aa) confirmed by MALDI-TOF
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Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation
Target
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