HSP70 1A Protein (AA 1-641) (His tag)
Quick Overview for HSP70 1A Protein (AA 1-641) (His tag) (ABIN666857)
Target
See all HSP70 1A (HSPA1A) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-641
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Purification tag / Conjugate
- This HSP70 1A protein is labelled with His tag.
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Sequence
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MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGD TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D -
Characteristics
- HSP70 (amino acids 1-641) Human, Recombinant, His- tagged, E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Heat Shock 70kDa Protein 1A (HSPA1A) (AA 291-641) protein (His-IF2DI Tag)
HSPA1A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Heat Shock 70kDa Protein 1A (HSPA1A) (Active) protein (His tag)HSPA1A Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE. Active
Heat Shock 70kDa Protein 1A (HSPA1A) (Active) protein (Myc-DYKDDDDK Tag)HSPA1A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 7.5) containing 2 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
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Alternative Name
- HSP70/HSPA1A
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Background
- Hsp70 is a human heat shock protein. Hsp70 is an important part of the cell's machinery for protein folding, and help to protect cells from stress. In most species, there are many proteins that belong to the hsp70 family. Some of these are only expressed under stress conditions, while some are present in cells under normal growth conditions and are not heat-inducible. They can be found in different cellular compartments (nuclear, cytosolic, mitochondrial, endoplasmic reticulum, etc... ). Recombinant human Hsp70, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 72.2 kDa (661aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_005336
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Target
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