TYMS Protein (AA 1-313) (His tag)

Catalog No. ABIN666924

This Recombinant TYMS protein is expressed in Escherichia coli (E. coli).

Quick Overview for TYMS Protein (AA 1-313) (His tag) (ABIN666924)

Target: TYMS (Thymidylate Synthetase (TYMS))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-313

Purification tag / Conjugate: This TYMS protein is labelled with His tag.

Sequence: MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV

Characteristics: Thymidylate synthase, 1-313aa, Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT, 0.1 M NaCl.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate synthase/TYMS

Background: Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu). Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 37.8 kDa (333aa) confirmed by MALDI-TOF

NCBI Accession: NP_001062

Pathways: Mitotic G1-G1/S Phases