HPRT1 Protein (AA 1-218) (His tag)

Catalog No. ABIN667016

This Recombinant HPRT1 protein is produced in Escherichia coli (E. coli).

Quick Overview for HPRT1 Protein (AA 1-218) (His tag) (ABIN667016)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS-PAGE

Product Details

Protein Characteristics: AA 1-218

Purification tag / Conjugate: This HPRT1 protein is labelled with His tag.

Characteristics: HPRT1, 1-218aa, Human, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing, 20% glycerol

Storage: 4 °C

Target Details

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HPRT1

Background: Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1. NCBI no.: NP_000185

Molecular Weight: 26.7 kDa (238aa), confirmed by MALDI-TOF

Pathways: Ribonucleoside Biosynthetic Process