PECI/ECI2 Protein (AA 1-364) (His tag)
Quick Overview for PECI/ECI2 Protein (AA 1-364) (His tag) (ABIN667028)
Target
See all PECI/ECI2 (PECI) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-364
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Purification tag / Conjugate
- This PECI/ECI2 protein is labelled with His tag.
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Sequence
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MNRTAMRASQ KDFENSMNQV KLLKKDPGNE VKLKLYALYK QATEGPCNMP KPGVFDLINK AKWDAWNALG SLPKEAARQN YVDLVSSLSP SLESSSQVEP GTDRKSTGFE TLVVTSEDGI TKIMFNRPKK KNAINTEMYH EIMRALKAAS KDDSIITVLT GNGDYYSSGN DLTNFTDIPP GGVEEKAKNN AVLLREFVGC FIDFPKPLIA VVNGPAVGIS VTLLGLFDAV YASDRATFHT PFSHLGQSPE GCSSYTFPKI MSPAKATEML IFGKKLTAGE ACAQGLVTEV FPDSTFQKEV WTRLKAFAKL PPNALRISKE VIRKREREKL HAVNAEECNV LQGRWLSDEC TNAVVNFLSR KSKL -
Characteristics
- PECI, 1-364aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Enoyl-CoA Delta Isomerase 2 (PECI) (AA 1-364) protein (GST tag)
PECI Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Enoyl-CoA Delta Isomerase 2 (PECI) (AA 31-394) protein (His-IF2DI Tag)PECI Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Enoyl-CoA Delta Isomerase 2 (PECI) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PECI Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Enoyl-CoA Delta Isomerase 2 (PECI) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PECI Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PECI/ECI2 (PECI) (Enoyl-CoA Delta Isomerase 2 (PECI))
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Alternative Name
- PECI/ECI2
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Background
- PECI, also referred to as DRS1, ACBD2 or HCA88, is a protein that localizes to the peroxisomal matrix and contains one ACB (acyl-CoA-binding) domain. This protein is expressed abundantly in liver, heart and skeletal muscle. It functions to catalyze the isomerization of both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species, playing an important role in the beta-oxidation of unsaturated fatty acids. Recombinant human PECI protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 42.3 kDa (384aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_006108
Target
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