WDR5 Protein (AA 1-334) (His tag)
Quick Overview for WDR5 Protein (AA 1-334) (His tag) (ABIN667153)
Target
See all WDR5 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-334
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Purification tag / Conjugate
- This WDR5 protein is labelled with His tag.
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Sequence
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MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS VKFSPNGEWL ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN LLVSASDDKT LKIWDVSSGK CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN RDGSLIVSSS YDGLCRIWDT ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL WDYSKGKCLK TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH TDVVISTACH PTENIIASAA LENDKTIKLW KSDC -
Characteristics
- WDR5, 1-334aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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WD Repeat Domain 5 (WDR5) (AA 1-334) protein (GST tag)
WDR5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
WD Repeat Domain 5 (WDR5) (AA 1-334) protein (His tag)WDR5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
WD Repeat Domain 5 (WDR5) (AA 2-334) protein (His-SUMO Tag)WDR5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
WD Repeat Domain 5 (WDR5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)WDR5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
WD Repeat Domain 5 (WDR5) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)WDR5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 2 mM DTT, 0.1M NaCl.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- WDR5 (WD Repeat Domain 5 (WDR5))
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Alternative Name
- WDR5
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Background
- WDR5, also designated BMP-2-induced gene 3 kb or BIG-3, belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. This protein is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. WDR5 may play a role in its function of accelerating osteoblast differentiation. Recombinant human WDR5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 38.8 kDa (354aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_438172
Target
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