NMT2 Protein (AA 1-498) (His tag)
Quick Overview for NMT2 Protein (AA 1-498) (His tag) (ABIN667348)
Target
See all NMT2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-498
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Purification tag / Conjugate
- This NMT2 protein is labelled with His tag.
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Sequence
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MAEDSESAAS QQSLELDDQD TCGIDGDNEE ETEHAKGSPG GYLGAKKKKK KQKRKKEKPN SGGTKSDSAS DSQEIKIQQP SKNPSVPMQK LQDIQRAMEL LSACQGPARN IDEAAKHRYQ FWDTQPVPKL DEVITSHGAI EPDKDNVRQE PYSLPQGFMW DTLDLSDAEV LKELYTLLNE NYVEDDDNMF RFDYSPEFLL WALRPPGWLL QWHCGVRVSS NKKLVGFISA IPANIRIYDS VKKMVEINFL CVHKKLRSKR VAPVLIREIT RRVNLEGIFQ AVYTAGVVLP KPIATCRYWH RSLNPKKLVE VKFSHLSRNM TLQRTMKLYR LPDVTKTSGL RPMEPKDIKS VRELINTYLK QFHLAPVMDE EEVAHWFLPR EHIIDTFVVE SPNGKLTDFL SFYTLPSTVM HHPAHKSLKA AYSFYNIHTE TPLLDLMSDA LILAKSKGFD VFNALDLMEN KTFLEKLKFG IGDGNLQYYL YNWRCPGTDS EKVGLVLQ -
Characteristics
- NMT2, 1-498aa, Human, His tag, E.coli
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Purification
- > 85% by SDS-PAGE
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N-Myristoyltransferase 2 (NMT2) (AA 1-529) protein (His tag)
custom-made NMT2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Myristoyltransferase 2 (NMT2) (AA 1-498) protein (His tag)custom-made NMT2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Myristoyltransferase 2 (NMT2) (AA 1-498) protein (GST tag)NMT2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
N-Myristoyltransferase 2 (NMT2) protein (Myc-DYKDDDDK Tag)NMT2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 2 mM DTT, 0.1M NaCl
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- NMT2 (N-Myristoyltransferase 2 (NMT2))
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Alternative Name
- NMT2
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Background
- NMT2, also known as glycylpeptide N-tetradecan-oyltransferases 2, is cytoplasmic protein that belong to the NMT family of proteins. The proteins in this familiy catalyze the addition of a myristoyl group to the N-terminal glycine residue of eukaryotic, fungal and viral proteins. They are primarily detected in heart, gut, kidney, liver and placenta. Recombinant human NMT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 59.1 kDa (518aa) confirmed by MALDI-TOF
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Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling
Target
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