FIMC (AA 37-241) protein (His tag)

Catalog No. ABIN667364

This Recombinant FIMC protein is produced in Escherichia coli (E. coli).

Quick Overview for FIMC (AA 37-241) protein (His tag) (ABIN667364)

Target: FIMC

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 37-241

Purification tag / Conjugate: His tag

Sequence: GVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME

Characteristics: eFIMC, 37-241aa, E.coli, His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: FIMC

Background: The 23 kDa two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages. Recombinant E. coli FIMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 25 kDa (226aa) confirmed by MALDI-TOF

NCBI Accession: NP_418736